Dr Adrian Biddle
DHT Lecturer in Animal Replacement Science
CPM organ-on-a-chip symposia organiser,
CPM Theme lead for Cancer Models
Centre for Cell Biology & Cutaneous Research, Blizard Institute
Faculty of Medicine and Dentistry, Queen Mary University of London
Faculty of Medicine and Dentistry, Queen Mary University of London
Research
cancer stem cells, tumour metastasis, 3D tumour invasion, metastasis on a chip, organ-on-a-chip, machine learning
Interests
Cancer stem cells drive tumour metastasis and therapeutic resistance. Understanding the underlying mechanisms will be crucial if we are to deliver better treatments for cancer, and we model this process using human cancer cell lines in advanced 3D invasion and microfluidic metastasis systems. We also use human tissue archives, which provide a wealth of information on tumour behaviour in the human body, including data on the clinical course of disease. We have developed new ways of obtaining greater information from these tumour specimens, including simultaneous measurement of multiple cancer stem cell markers and a machine learning approach for prognostic prediction.Publications
Publications of specific relevance to Predictive in vitro Models2024
Scemama A, Lunetto S, Tailor A, Di Cio S, Dibble M, Gautrot J and Biddle A (2024). Development of an in vitro microfluidic model to study the role of microenvironmental cells in oral cancer metastasis. F1000Research F1000Research vol. 12, 2023
Youssef G, Gammon L, Ambler L, Lunetto S, Scemama A, Cottom H, Piper K, Mackenzie IC, Philpott MP and Biddle A (2023). Disseminating cells in human oral tumours possess an EMT cancer stem cell marker profile that is predictive of metastasis in image-based machine learning. eLife eLife vol. 12, Samain R, Maiques O, Monger J, Lam H, Candido J, George S, Ferrari N, KohIhammer L, Lunetto S, Varela A, Orgaz JL, Vilardell F, Olsina JJ, Matias-Guiu X, Sarker D, Biddle A, Balkwill FR, Eyles J, Wilkinson RW, Kocher HM, Calvo F, Wells CM and Sanz-Moreno V (2023). CD73 controls Myosin II–driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells. American Association for the Advancement of Science (AAAS) Science Advances vol. 9, (42) Scemama A, Tailor A, Di Cio S, Dibble M, Gautrot J and Biddle A (2023). Development of an in vitro microfluidic model to study the role of microenvironmental cells in oral cancer metastasis. Taylor and Francis F1000Research vol. 12, 439-439. Biddle A (2023). In vitro cancer models as an approach to identify targetable developmental phenotypes in cancer stem cells. Springer Nature In vitro models vol. 2, (3-4) 83-88. 2022
Biddle A (2022). Organ-on-a-Chip Technologies Network special issue editorial. Springer Nature In vitro models vol. 1, (6) 397-398. Marles H and Biddle A (2022). Cancer stem cell plasticity and its implications in the development of new clinical approaches for oral squamous cell carcinoma. Elsevier Biochemical Pharmacology vol. 204, Scemama A, Lunetto S and Biddle A (2022). Highlight: microfluidic devices for cancer metastasis studies. Springer Nature In vitro models vol. 1, (6) 399-403. Sahoo S, Ashraf B, Duddu AS, Biddle A and Jolly MK (2022). Interconnected high-dimensional landscapes of epithelial–mesenchymal plasticity and stemness in cancer. Springer Nature Clinical & Experimental Metastasis vol. 39, (2) 279-290. 2020
Custódio M, Biddle A and Tavassoli M (2020). Portrait of a CAF: The story of cancer-associated fibroblasts in head and neck cancer. Elsevier Oral Oncology vol. 110, 2018
Jolly MK, Somarelli JA, Sheth M, Biddle A, Tripathi SC, Armstrong AJ, Hanash SM, Bapat SA, Rangarajan A and Levine H (2018). Hybrid epithelial/mesenchymal phenotypes promote metastasis and therapy resistance across carcinomas. Elsevier Pharmacology & Therapeutics vol. 194, 161-184. MACKENZIE IC, Rodrigues MF, Gammon L, RAHMAN M, BIDDLE A and Nunes FD (2018). Effects of Cetuximab and Erlotinib on the behaviour of cancer stem cells in head and neck squamous cell carcinoma. Impact Journals Oncotarget 2017
O’Brien-Ball C and Biddle A (2017). Reprogramming to developmental plasticity in cancer stem cells. Elsevier Developmental Biology vol. 430, (2) 266-274. 2016
BIDDLE A, Gammon L, Liang X, Costea DE and Mackenzie IC (2016). Phenotypic Plasticity Determines Cancer Stem Cell Therapeutic Resistance in Oral Squamous Cell Carcinoma. Elsevier: Creative Commons EBioMedicine 2015
Gemenetzidis E, Gammon L, Biddle A, Emich H and Mackenzie IC (2015). Invasive oral cancer stem cells display resistance to ionising radiation. Impact Journals Oncotarget vol. 6, (41) 43964-43977. Shigeishi H, Biddle A, Gammon L, Rodini CO, Yamasaki M, Seino S, Sugiyama M, Takechi M and Mackenzie IC (2015). Elevation in 5-FU-induced apoptosis in head and neck cancer stem cells by a combination of CDHP and GSK3β inhibitors. J Oral Pathol Med vol. 44, (3) 201-207. Vig N, Mackenzie IC and Biddle A (2015). Phenotypic plasticity and epithelial‐to‐mesenchymal transition in the behaviour and therapeutic response of oral squamous cell carcinoma. Wiley Journal of Oral Pathology and Medicine vol. 44, (9) 649-655. 2014
Vig N, Biddle A, Gammon L, Emich H and Mackenzie I (2014). Implications of the Cancer Stem Cell (CSC) Paradigm for OMFS Patients and Surgeons. Elsevier British Journal of Oral and Maxillofacial Surgery vol. 52, (8) 2013
Shigeishi H, Biddle A, Gammon L, Emich H, Rodini CO, Gemenetzidis E, Fazil B, Sugiyama M, Kamata N and Mackenzie IC (2013). Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3β influenced by CD44 and RHAMM. Stem Cells vol. 31, (10) 2073-2083. Biddle A, Gammon L, Fazil B and Mackenzie IC (2013). CD44 staining of cancer stem-like cells is influenced by down-regulation of CD44 variant isoforms and up-regulation of the standard CD44 isoform in the population of cells that have undergone epithelial-to-mesenchymal transition. PLoS One vol. 8, (2) Gammon L, Biddle A, Heywood HK, Johannessen AC and Mackenzie IC (2013). Sub-sets of cancer stem cells differ intrinsically in their patterns of oxygen metabolism. PLoS One vol. 8, (4) Hufbauer M, Biddle A, Borgogna C, Gariglio M, Doorbar J, Storey A, Pfister H, Mackenzie I and Akguel B (2013). Expression of Betapapillomavirus Oncogenes Increases the Number of Keratinocytes with Stem Cell-Like Properties. JOURNAL OF VIROLOGY vol. 87, (22) 12158-12165. 2012
Biddle A and Mackenzie IC (2012). Cancer stem cells and EMT in carcinoma. Cancer Metastasis Rev VALORANI MG, Montelatici E, Germani A, Biddle A, D'Alessandro D, Strollo R, Patrizi MP, Lazzari L, Nye E and Otto WR (2012). Pre-culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potential. Cell Proliferation vol. 45, (3) 225-238. 2011
Biddle A, Liang X, Gammon L, Fazil B, Harper LJ, Emich H, Costea DE and Mackenzie IC (2011). Cancer stem cells in squamous cell carcinoma switch between two distinct phenotypes that are preferentially migratory or proliferative. Cancer Res vol. 71, (15) 5317-5326. Gammon L, Biddle A, Fazil B, Harper L and Mackenzie IC (2011). Stem cell characteristics of cell sub-populations in cell lines derived from head and neck cancers of Fanconi anemia patients. J Oral Pathol Med vol. 40, (2) 143-152. 2010
Valorani MG, Germani A, Otto WR, Harper L, Biddle A, Khoo CP, Lin WR, Hawa MI, Tropel P, Patrizi MP, Pozzilli P and Alison MR (2010). Hypoxia increases Sca-1/CD44 co-expression in murine mesenchymal stem cells and enhances their adipogenic differentiation potential. Cell Tissue Res vol. 341, (1) 111-120. Harper LJ, Costea DE, Gammon L, Fazil B, Biddle A and Mackenzie IC (2010). Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance. BMC Cancer vol. 10, 2009
Biddle A, Simeoni I and Gurdon JB (2009). Xenopus oocytes reactivate muscle gene transcription in transplanted somatic nuclei independently of myogenic factors. DEVELOPMENT vol. 136, (16) 2695-2703. 2005
Dragnev KH, Petty WJ, Shah S, Biddle A, Desai NB, Memoli V, Rigas JR and Dmitrovsky E (2005). Bexarotene and Erlotinib for Aerodigestive Tract Cancer. American Society of Clinical Oncology (ASCO) Journal of Clinical Oncology vol. 23, (34) 8757-8764. Petty WJ, Li N, Biddle A, Bounds R, Nitkin C, Ma Y, Dragnev KH, Freemantle SJ and Dmitrovsky E (2005). A Novel Retinoic Acid Receptor β Isoform and Retinoid Resistance in Lung Carcinogenesis. Oxford University Press (OUP) Journal of the National Cancer Institute vol. 97, (22) 1645-1651. 2004
Petty WJ, Dragnev KH, Memoli VA, Ma Y, Desai NB, Biddle A, Davis TH, Nugent WC, Memoli N, Hamilton M, Iwata KK, Rigas JR and Dmitrovsky E (2004). Epidermal Growth Factor Receptor Tyrosine Kinase Inhibition Represses Cyclin D1 in Aerodigestive Tract Cancers. American Association for Cancer Research (AACR) Clinical Cancer Research vol. 10, (22) 7547-7554. Grants
Grants of specific relevance to Predictive in vitro Models(EPSRC CDT 2023) Next Generation Organ-on-a-Chip TechnologiesHazel Screen, Martin Knight, Thomas Iskratsch, Caroline Roney, Yi Sui, David Lee, Julia Shelton, Tsz Ho Tse, John Connelly, Adrian Biddle and Lucy Norling
£7,066,811 EPSRC Engineering and Physical Sciences Research Council (01-07-2024 - 30-09-2032)
PhD Study 2022 – Modelling the tumour-induced changes to the tumour microenvironment (TME) and extracellular matrix (ECM) within a defined minimal 3D in vitro environment.Adrian Biddle
£50,000 Animal Free Research UK (06-06-2022 - 05-06-2025)
Identifying and manipulating molecular mechanisms controlling cancer stem cell metastatic potential in a human oral cancer model.£552,481 MRC Medical Research Council (01-09-2021 - 30-06-2025)
The molecular mechanisms underlying the tumour-determined immune cell infiltrate in oral squamous cell carcinomaAdrian Biddle and Muy-Teck Teh
£272,996 MCSBHT Medical College of St Bartholomew's Hospital Trust (01-09-2021 - 31-12-2025)