Dr Matthew Caley
Senior Lecturer in Cell Biology
Research
Skin, Lipids, rare diseases, screening, Extracellular Matrix, Laminin 332
Interests
My research focusses on the skin basement membrane and specifically laminin 332 a three-chained glycoprotein which is copiously secreted by epithelial cells. We have demonstrated an important role for laminin 332 in skin ageing, squamous cell carcinoma invasion, cholesterol transport and in skin immune regulation. To study these diverse functions we have generated co-culture and 3D skin models including models of aged skin that have allowed us to test gene knockdowns and drug treatments. We have also developed and validated a high throughput screen to identify compounds altering cholesterol transport within the skin.Publications
Publications of specific relevance to Predictive in vitro Models2024
Jones EM, Garcha P, Aumailley M, O’Toole EA, Rognoni E and Caley M (2024). Weekly Intraperitoneal Injection of Tamoxifen in an Inducible in vivo Model of Junctional Epidermolysis Bullosa Generates Early and Advanced Disease Phenotypes. Elsevier JID Innovations Quílez C, Bebiano LB, Jones E, Maver U, Meesters L, Parzymies P, Petiot E, Rikken G, Risueño I, Zaidi H, Zidarič T, Bekeschus S, H van den Bogaard E, Caley M, Colley H, López NG, Letsiou S, Marquette C, Maver T, Pereira RF, Tobin DJ and Velasco D (2024). Targeting the Complexity of In Vitro Skin Models: A Review of Cutting-Edge Developments. Elsevier Journal of Investigative Dermatology vol. 144, (12) 2650-2670. Tawfik S, Thomas B, Caley M, Jones E, O’Shaughnessy R and O’Toole E (2024). P038 Impact of cumulative low-level ozone on keratinocytes and implications for atopic eczema pathogenesis. Oxford University Press (OUP) British Journal of Dermatology vol. 191, (Supplement_1) i31-i32. Khan MRA, Kirk T, Caley MP and Rognoni E (2024). P16 Fibroblast signalling crosstalk drives junctional epidermolysis bullosa disease progression. Oxford University Press (OUP) British Journal of Dermatology vol. 190, (6) e86-e86. Giusto E, Jones E, Enjalbert F, Ahmed A, Caley M, Rognoni E and O’Toole E (2024). P10 Janus kinase inhibitors to restore skin barrier function in a mouse model of Harlequin ichthyosis. Oxford University Press (OUP) British Journal of Dermatology vol. 190, (6) e83-e84. Garcha P, Jones E, Ahmed AA, Aumailley M, Rognoni E and Caley M (2024). O20 Dissecting junctional epidermolysis bullosa heterogeneity. Oxford University Press (OUP) British Journal of Dermatology vol. 190, (6) e77-e78. Jones E, Garcha P, Aumailley M, O’Toole E, Rognoni E and Caley M (2024). O19 Improvement of a junctional epidermolysis bullosa mouse model. Oxford University Press (OUP) British Journal of Dermatology vol. 190, (6) e77-e77. Summan S, Mayes A, Gunn D, Madapura P, Gautrot J, Caley M and O’Toole E (2024). O08 Exploring the effect of skin ageing-related basement membrane collagen loss on nuclear mechanics. Oxford University Press (OUP) British Journal of Dermatology vol. 190, (6) e71-e72. Guri-Lamce I, Alrokh Y, Graham C, Maeshima R, Rognoni E, Caley M, Łaczmański Ł, Hart SL, McGrath JA and Jacków-Malinowska J (2024). Lipid Nanoparticles Efficiently Deliver the Base Editor ABE8e for COL7A1 Correction in Dystrophic Epidermolysis Bullosa Fibroblasts In Vitro. Elsevier Journal of Investigative Dermatology vol. 144, (10) 2314-2317.e3. 2023
Caley M, Idrissi M, Braiman L, Brener E and O'Toole E (2023). LB1754 Treatment of human palmoplantar keratoderma skin equivalents with a TRPV3 antagonist, KM-001. Elsevier Journal of Investigative Dermatology vol. 143, (9) Garcha P, Jones E, Shelley J and Caley M (2023). P32 Restoring the ageing skin barrier in vitro. Oxford University Press (OUP) British Journal of Dermatology vol. 189, (1) e26-e27. Parzymies P, Jones EM, Mayes A, Messenger D, O’Toole E and Caley M (2023). O16 The novel roles of laminin α3 in epidermal barrier function and metabolism. Oxford University Press (OUP) British Journal of Dermatology vol. 189, (1) e11-e11. Giusto E, Enjalbert F, Rognoni E, Caley M and O’Toole E (2023). O13 JAK inhibitors to restore skin barrier function in ARCI. Oxford University Press (OUP) British Journal of Dermatology vol. 189, (1) e9-e10. Summan SK, Mayes A, Gunn D, Gautrot J, Caley M and O’Toole E (2023). P16 How does skin ageing-related basement membrane collagen loss affect the nucleus? Oxford University Press (OUP) British Journal of Dermatology vol. 189, (1) e20-e20. Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath J, O’Toole E and Caley M (2023). O14 Junctional epidermolysis bullosa: repairing the epidermal lipid barrier. Oxford University Press (OUP) British Journal of Dermatology vol. 189, (1) e10-e10. Lohana P, Suryaprawira A, Woods EL, Dally J, Gait-Carr E, Alaidaroos NYA, Heard CM, Lee KY, Ruge F, Farrier JN, Enoch S, Caley MP, Peake MA, Davies LC, Giles PJ, Thomas DW, Stephens P and Moseley R (2023). Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues. MDPI Antioxidants vol. 12, (7) McGeoghan F, Camera E, Maiellaro M, Menon M, Huang M, Dewan P, Ziaj S, Caley MP, Donaldson M, Enright AJ and O’Toole EA (2023). RNA sequencing and lipidomics uncovers novel pathomechanisms in recessive X-linked ichthyosis. Frontiers Frontiers in Molecular Biosciences vol. 10, Hindle S, Taggart E, Blowes L, Pennington D, Singh B, Caley M and Connelly J (2023). 1035 Understanding dynamic immune responses within a 3D microfluidic model of human skin. Elsevier Journal of Investigative Dermatology vol. 143, (5) Tawfik SS, Thomas B, Jones E, Caley M, O'Shaughnessy R and O'Toole E (2023). 1157 Ozone as an aggravating factor in atopic eczema. Elsevier Journal of Investigative Dermatology vol. 143, (5) Parzymies PJ, Jones E, Mayes A, Messenger D, O'Toole E and Caley M (2023). 763 Novel roles of laminin α3 in epidermal homeostasis. Elsevier Journal of Investigative Dermatology vol. 143, (5) Jones E, Marsh S, Aumailley M, McGrath JA, O'Toole E, Rognoni E and Caley M (2023). 753 Repairing the epidermal skin barrier in an in vivo model of junctional epidermolysis bullosa. Elsevier Journal of Investigative Dermatology vol. 143, (5) Summan SK, Mayes A, Gunn DA, Gautrot J, Caley M and O'Toole E (2023). 741 The effect of skin ageing-related basement membrane collagen loss on the nucleus. Elsevier Journal of Investigative Dermatology vol. 143, (5) s127-s127. Tawfik S, O'Toole E, Thomas B, Caley M, Jones E and O'Shaughnessy R (2023). ISID1157 - Ozone as an aggravating factor in atopic eczema. Morressier Frost JM, Amante SM, Okae H, Jones EM, Ashley B, Lewis RM, Cleal JK, Caley MP, Arima T, Maffucci T and Branco MR (2023). Regulation of human trophoblast gene expression by endogenous retroviruses. Springer Nature Nature Structural & Molecular Biology vol. 30, (4) 527-538. 
Smith CJ, Williams JL, Hall C, Casas J, Caley MP, O'Toole EA, Prasad R and Metherell LA (2023). Ichthyosis linked to sphingosine 1-phosphate lyase insufficiency is due to aberrant sphingolipid and calcium regulation. Elsevier Journal of Lipid Research vol. 64, (4) 2022
Dina DO, Caley MP, Raj N, O’Toole EA and Connelly J (2022). 216 Understanding the Mechanisms of Epidermal Barrier Damage and Repair Using 3D Skin Equivalent Models. Elsevier Journal of Investigative Dermatology vol. 142, (12) Jones E, Marsh S, O’Shaughnessy R, Aumailley M, McGrath JA, O’Toole EA and Caley MP (2022). 191 Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier. Elsevier Journal of Investigative Dermatology vol. 142, (12) Tawfik SS, Thomas B, Jones E, Caley MP, O’Shaughnessy R and O’Toole EA (2022). 144 Carbonylated proteins and Ozone in atopic eczema. Elsevier Journal of Investigative Dermatology vol. 142, (12) Parzymies P, Jones E, Caley MP, O’Toole EA, Messenger D and Mayes A (2022). 184 The role of the Laminin 332 in cholesterol transport in dry and Aged skin. Elsevier Journal of Investigative Dermatology vol. 142, (12) Smith CJ, Williams JL, Hall C, Caley MP, O’Toole EA, Prasad R and Metherell LA (2022). 181 Sphingosine 1-phosphate lyase insufficiency induced ichthyosis is due to sphingolipid imbalance, increased differentiation, and aberrant intercellular junctions. Elsevier Journal of Investigative Dermatology vol. 142, (12) Tawfik S, Thomas B, Jones E, Caley M, O'Shaughnessy R, O'Toole E and Prasad Baruah K (2022). ESDR144 - Carbonylated proteins and Ozone in atopic eczema. Morressier Dina D, Caley M, Raj N, O'Toole E and Connelly J (2022). ESDR216 - Understanding the Mechanisms of Epidermal Barrier Damage and Repair Using 3D Skin Equivalent Models. Morressier Parzymies P, Jones E, Caley M, O'Toole E, Messenger D and Mayes A (2022). ESDR184 - The role of the Laminin 332 in cholesterol transport in dry and Aged skin. Morressier Smith C, Williams J, Hall C, Caley M, O'Toole E, Prasad R and Metherell L (2022). ESDR181 - Sphingosine 1-phosphate lyase insufficiency induced ichthyosis is due to sphingolipid imbalance, increased differentiation, and aberrant intercellular junctions. Morressier Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath J, O'Toole E and Caley M (2022). ESDR191 - Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier. Morressier Smith CJ, Parkinson EK, Yang J, Pratten J, O'Toole EA, Caley MP and Braun KM (2022). Investigating wound healing characteristics of gingival and skin keratinocytes in organotypic cultures. Elsevier Journal of Dentistry vol. 125, 2021
Smith CJ, Williams JL, Caley MP, O’Toole EA, Prasad R and Metherell LA (2021). 099 Ichthyosis associated with sphingosine 1-phosphate lyase deficiency is due to aberrant calcium and sphingolipid regulation. Journal of Investigative Dermatology vol. 141, (10) s165-s165. Caley MP, Martins VL, Moore K, Lashari M, Nissinen L, Kähäri V-M, Alexander S, Jones E, Harwood CA, Jones J, Donaldson M, Marshall JF and O'Toole EA (2021). Loss of the laminin subunit alpha-3 induces cell invasion and macrophage infiltration in cutaneous squamous cell carcinoma. Br J Dermatol vol. 184, (5) 923-934. 2020
Langton AK, Ayer J, Griffiths TW, Rashdan E, Naidoo K, Caley MP, Birch‐Machin MA, O'Toole EA, Watson REB and Griffiths CEM (2020). Distinctive clinical and histological characteristics of atrophic and hypertrophic facial photoageing. Wiley Journal of the European Academy of Dermatology and Venereology vol. 35, (3) 762-768. Enjalbert F, Dewan P, Caley MP, Jones EM, Morse MA, Kelsell DP, Enright AJ and O'Toole EA (2020). 3D model of harlequin ichthyosis reveals inflammatory therapeutic targets. American Society for Clinical Investigation Journal of Clinical Investigation 2019
Caley M and Mai Jones E (2019). Junctional Epidermolysis Bullosa: Bottom Up Control Of The Skin Barrier? Morressier Hassan S, Purdie KJ, Wang J, Harwood CA, Proby CM, Pourreyron C, Mladkova N, Nagano A, Dhayade S, Athineos D, Caley M, Mannella V, Blyth K, Inman GJ and Leigh IM (2019). A Unique Panel of Patient-Derived Cutaneous Squamous Cell Carcinoma Cell Lines Provides a Preclinical Pathway for Therapeutic Testing. MDPI International Journal of Molecular Sciences vol. 20, (14) 2018
Caley M, Wall IB, Peake M, Kipling D, Giles P, Thomas DW and Stephens P (2018). Development and Characterisation of a Human Chronic Skin Wound Cell Line—Towards an Alternative for Animal Experimentation. MDPI International Journal of Molecular Sciences vol. 19, (4) 2016
Branco MR, King M, Perez-Garcia V, Bogutz AB, Caley M, Fineberg E, Lefebvre L, Cook SJ, Dean W, Hemberger M and Reik W (2016). Maternal DNA Methylation Regulates Early Trophoblast Development. Elsevier Developmental Cell vol. 36, (2) 152-163. 2015
Caley MP, King H, Shah N, Wang K, Rodriguez-Teja M, Gronau JH, Waxman J and Sturge J (2015). Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces. Springer Nature Clinical & Experimental Metastasis vol. 33, (2) 151-165. Hill DS, Robinson NDP, Caley MP, Chen M, O'Toole EA, Armstrong JL, Przyborski S and Lovat PE (2015). A Novel Fully Humanized 3D Skin Equivalent to Model Early Melanoma Invasion. American Association for Cancer Research (AACR) Molecular Cancer Therapeutics vol. 14, (11) 2665-2673. Kopecki Z, Yang GN, Jackson JE, Melville EL, Calley MP, Murrell DF, Darby IA, O'Toole EA, Samuel MS and Cowin AJ (2015). Cytoskeletal protein Flightless I inhibits apoptosis, enhances tumor cell invasion and promotes cutaneous squamous cell carcinoma progression. Impact Journals Oncotarget vol. 6, (34) 36426-36440. Martins VL, Caley MP, Moore K, Szentpetery Z, Marsh ST, Murrell DF, Kim MH, Avari M, McGrath JA, Cerio R, Kivisaari A, Kähäri VM, Hodivala-Dilke K, Brennan CH, Chen M, Marshall JF and O'Toole EA (2015). Suppression of TGFβ and Angiogenesis by Type VII Collagen in Cutaneous SCC. J Natl Cancer Inst vol. 108, (1) Caley MP, Martins VLC and O'Toole EA (2015). Metalloproteinases and Wound Healing. Mary Ann Liebert Advances in Wound Care vol. 4, (4) 225-234. 2014
Rodriguez‐Teja M, Gronau JH, Breit C, Zhang YZ, Minamidate A, Caley MP, McCarthy A, Cox TR, Erler JT, Gaughan L, Darby S, Robson C, Mauri F, Waxman J and Sturge J (2014). AGE‐modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival. Wiley The Journal of Pathology vol. 235, (4) 581-592. Cichoń MA, Szentpetery Z, Caley MP, Papadakis ES, Mackenzie IC, Brennan CH and O'Toole EA (2014). The receptor tyrosine kinase Axl regulates cell-cell adhesion and stemness in cutaneous squamous cell carcinoma. Oncogene vol. 33, (32) 4185-4192. Peake MA, Caley M, Giles PJ, Wall I, Enoch S, Davies LC, Kipling D, Thomas DW and Stephens P (2014). Identification of a transcriptional signature for the wound healing continuum. Wound Repair Regen vol. 22, (3) 399-405. 2013
Pellegrino L, Stebbing J, Braga VM, Frampton AE, Jacob J, Buluwela L, Jiao LR, Periyasamy M, Madsen CD, Caley MP, Ottaviani S, Roca-Alonso L, El-Bahrawy M, Coombes RC, Krell J and Castellano L (2013). miR-23b regulates cytoskeletal remodeling, motility and metastasis by directly targeting multiple transcripts. Nucleic Acids Res vol. 41, (10) 5400-5412. Martins VL, Caley M and O'Toole EA (2013). Matrix metalloproteinases and epidermal wound repair. Cell Tissue Res vol. 351, (2) 255-268. Palmieri C, Caley MP, Purshouse K, Fonseca A-V, Rodriguez-Teja M, Kogianni G, Woodley L, Odendaal J, Elliott K, Waxman J and Sturge J (2013). Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer. Br J Cancer vol. 108, (1) 163-169. Stephens P, Caley M and Peake M (2013). Alternatives for animal wound model systems. Methods Mol Biol vol. 1037, 177-201. Stephens P, Caley M and Peake M (2013). Alternatives for animal wound model systems. Methods in Molecular Biology vol. 1037, 177-201. 2012
Ramasamy K, Khatun H, Macpherson L, Caley MP, Sturge J, Mufti GJ, Schey SA and Calle Y (2012). Fluorescence-based experimental model to evaluate the concomitant effect of drugs on the tumour microenvironment and cancer cells. Br J Haematol vol. 157, (5) 564-579. Caley MP, Kogianni G, Adamarek A, Gronau JH, Rodriguez-Teja M, Fonseca A-V, Mauri F, Sandison A, Rhim JS, Pchejetski D, Palmieri C, Cobb JP, Waxman J and Sturge J (2012). TGFβ1-Endo180-dependent collagen deposition is dysregulated at the tumour-stromal interface in bone metastasis. J Pathol vol. 226, (5) 775-783. 2011
Sturge J, Caley MP and Waxman J (2011). Bone metastasis in prostate cancer: emerging therapeutic strategies. Nat Rev Clin Oncol vol. 8, (6) 357-368. Sturge J, Caley MP and Waxmanin J (2011). Authors' reply: Expanding horizons in metastatic prostate cancer treatment. NATURE REVIEWS CLINICAL ONCOLOGY vol. 8, (10) Sturge J, Caley MP and Waxman J (2011). Bone metastasis in prostate cancer: emerging therapeutic strategies (vol 8, pg 357, 2011). NATURE REVIEWS CLINICAL ONCOLOGY vol. 8, (10) 568-568. 2008
Mantripragada KK, Caley M, Stephens P, Jones CJ, Kluwe L, Guha A, Mautner V and Upadhyaya M (2008). Telomerase activity is a biomarker for high grade malignant peripheral nerve sheath tumors in neurofibromatosis type 1 individuals. Genes Chromosomes Cancer vol. 47, (3) 238-246. Mantripragada KK, Caley M, Stephens P, Jones CJ, Kluwe L, Guha A, Mautner V and Upadhyaya M (2008). Telomerase activity is a biomarker for high grade malignant peripheral nerve sheath tumors in neurofibromatosis type I individuals. GENES CHROMOSOMES & CANCER vol. 47, (3) 238-246. 2007
Caley M, Wall I, Kipling D, Thomas DW and Stephens P (2007). Development and characterisation of a human cell-based chronic wound bioassay. WOUND REPAIR AND REGENERATION vol. 15, (6) A133-A133. Grants
Dissecting The Role Of Inflammatory pathways In Epidermolysis Bullosa (EB) PathologyEmma Chambers, Emanuel Rognoni and Matthew Caley
£139,912 DEBRA UK (01-04-2024 - 31-03-2028)
The role of the Laminin 332-Actin-Cholesterol axis in skin conditions with altered lipid profiles£64,000 Unilever UK Ltd (01-10-2020 - 30-06-2025)