Dr Pradeepa Madapura
Senior Lecturer
Blizard Institute
Faculty of Medicine and Dentistry
Faculty of Medicine and Dentistry
Research
Neurodevelopmental disorders, brain organoids, In vitro brain development, neuroepigenetics, functional genomics
Interests
We use human stem cell-derived cerebral organoid models to investigate the mechanism through which mutations in epigenetic regulators contribute to neurodevelopmental and cardiovascular disorders.Publications
2024
Jayakumar S, Patel M, Boulet F, Aziz H, Brooke GN, Tummala H and Pradeepa MM (2024). Author Correction: PSIP1/LEDGF reduces R-loops at transcription sites to maintain genome integrity. Springer Nature Nature Communications vol. 15, (1)
Jacob DR, Guiblet WM, Mamayusupova H, Shtumpf M, Ciuta I, Ruje L, Gretton S, Bikova M, Correa C, Dellow E, Agrawal SP, Shafiei N, Drobysevskaja A, Armstrong CM, Lam JDG, Vainshtein Y, Clarkson CT, Thorn GJ, Sohn K, Pradeepa MM, Chandrasekharan S, Brooke GN, Klenova E, Zhurkin VB and Teif VB (2024). Nucleosome reorganisation in breast cancer tissues. Springer Nature Clinical Epigenetics vol. 16, (1)
Jayakumar S, Patel M, Boulet F, Aziz H, Brooke GN, Tummala H and Pradeepa MM (2024). PSIP1/LEDGF reduces R-loops at transcription sites to maintain genome integrity. Springer Nature Nature Communications vol. 15, (1)
2023
Pal D, Patel M, Boulet F, Sundarraj J, Grant OA, Branco MR, Basu S, Santos SDM, Zabet NR, Scaffidi P and Pradeepa MM (2023). H4K16ac activates the transcription of transposable elements and contributes to their cis-regulatory function. Springer Nature Nature Structural & Molecular Biology vol. 30, (7) 935-947.
De Vas MG, Boulet F, Joshi SS, Garstang MG, Khan TN, Atla G, Parry D, Moore D, Cebola I, Zhang S, Cui W, Lampe AK, Lam WW, Consortium GER, Ferrer J, Pradeepa MM and Atanur SS (2023). Regulatory de novo mutations underlying intellectual disability. Life Science Alliance Life Science Alliance vol. 6, (5)
2022
Rodriguez-Algarra F, Seaborne RAE, Danson AF, Yildizoglu S, Yoshikawa H, Law PP, Ahmad Z, Maudsley VA, Brew A, Holmes N, Ochôa M, Hodgkinson A, Marzi SJ, Pradeepa MM, Loose M, Holland ML and Rakyan VK (2022). Genetic variation at mouse and human ribosomal DNA influences associated epigenetic states. Springer Nature Genome Biology vol. 23, (1)
2021
Sundarraj J, Taylor GCA, von Kriegsheim A and Pradeepa MM (2021). H3K36me3 and PSIP1/LEDGF associate with several DNA repair proteins, suggesting their role in efficient DNA repair at actively transcribing loci. F1000Research Wellcome Open Research vol. 2,
Olley G, Pradeepa MM, Grimes GR, Piquet S, Polo SE, FitzPatrick DR, Bickmore WA and Boumendil C (2021). Cornelia de Lange syndrome-associated mutations cause a DNA damage signalling and repair defect. Springer Nature Nature Communications vol. 12, (1)
2019
Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park S-M, Bickmore WA, Pradeepa MM and FitzPatrick DR (2019). Publisher Correction: BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Springer Nature Nature Genetics vol. 51, (7) 1192-1192.
2018
Garstang MG and Pradeepa MM (2018). An Enhancer-Derived RNA Muscles In to Regulate Myogenin In trans. Mol Cell vol. 71, (1) 3-5.
Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park S-M, Bickmore WA, Pradeepa MM, FitzPatrick DR and Deciphering Developmental Disorders Study (2018). Publisher Correction: BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Springer Nature Nature Genetics vol. 50, (5) 767-767.
Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park S-M, Deciphering Developmental Disorders Study , Bickmore WA, Pradeepa MM and FitzPatrick DR (2018). BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Springer Nature Nature Genetics vol. 50, (3) 329-332.
2017
Marin R, Cortez D, Lamanna F, Pradeepa MM, Leushkin E, Julien P, Liechti A, Halbert J, Brüning T, Mössinger K, Trefzer T, Conrad C, Kerver HN, Wade J, Tschopp P and Kaessmann H (2017). Convergent origination of a Drosophila-like dosage compensation mechanism in a reptile lineage. Cold Spring Harbor Laboratory Genome Research vol. 27, (12) 1974-1987.
Pradeepa MM, Taylor GCA and von Kriegsheim A (2017). Proteomic analysis of H3K36me3 and PSIP1/p75 (LEDGF) complexes reveal their wider role in DNA repair. F1000Research Wellcome Open Research vol. 2,
Pradeepa MM, McKenna F, Taylor GCA, Bengani H, Grimes GR, Wood AJ, Bhatia S and Bickmore WA (2017). Psip1/p52 regulates posterior Hoxa genes through activation of lncRNA Hottip. Public Library of Science (PLoS) PLOS Genetics vol. 13, (4)
Pradeepa MM (2017). Causal role of histone acetylations in enhancer function. Transcription vol. 8, (1) 40-47.
2016
Marulasiddappa P, Pradeepa MM, Kumar Y, Grimes G, Illingworth R and Bickmore W (2016). Native chromatin immunoprecipitation of histone marks to Identify enhancer status. Springer Nature Protocol Exchange
Pradeepa MM, Grimes GR, Kumar Y, Olley G, Taylor GCA, Schneider R and Bickmore WA (2016). Histone H3 globular domain acetylation identifies a new class of enhancers. Nature Genetics vol. 48, (6) 681-686.
Robertson KA, Hsieh WY, Forster T, Blanc M, Lu H, Crick PJ, Yutuc E, Watterson S, Martin K, Griffiths SJ, Enright AJ, Yamamoto M, Pradeepa MM, Lennox KA, Behlke MA, Talbot S, Haas J, Dölken L, Griffiths WJ, Wang Y, Angulo A and Ghazal P (2016). An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway. Public Library of Science (PLoS) PLOS Biology vol. 14, (3)
2015
Illingworth RS, Moffat M, Mann AR, Read D, Hunter CJ, Pradeepa MM, Adams IR and Bickmore WA (2015). The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development. Cold Spring Harbor Laboratory Genes & Development vol. 29, (18) 1897-1902.
Gupta N, Madapura MP, Bhat UA and Rao MRS (2015). Mapping of Post-translational Modifications of Transition Proteins, TP1 and TP2, and Identification of Protein Arginine Methyltransferase 4 and Lysine Methyltransferase 7 as Methyltransferase for TP2*. Elsevier Journal of Biological Chemistry vol. 290, (19) 12101-12122.
2014
Pradeepa MM, Grimes GR, Taylor GCA, Sutherland HG and Bickmore WA (2014). Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Oxford University Press (OUP) Nucleic Acids Research vol. 42, (14) 9021-9032.
2013
Taylor GCA, Eskeland R, Hekimoglu-Balkan B, Pradeepa MM and Bickmore WA (2013). H4K16 acetylation marks active genes and enhancers of embryonic stem cells, but does not alter chromatin compaction. Cold Spring Harbor Laboratory Genome Research vol. 23, (12) 2053-2065.
2012
Pradeepa MM, Sutherland HG, Ule J, Grimes GR and Bickmore WA (2012). Psip1/Ledgf p52 Binds Methylated Histone H3K36 and Splicing Factors and Contributes to the Regulation of Alternative Splicing. Public Library of Science (PLoS) PLOS Genetics vol. 8, (5)
2009
Pradeepa MM, Nikhil G, Kishore AH, Bharath GN, Kundu TK and Rao MRS (2009). Acetylation of Transition Protein 2 (TP2) by KAT3B (p300) Alters Its DNA Condensation Property and Interaction with Putative Histone Chaperone NPM3*. Elsevier Journal of Biological Chemistry vol. 284, (43) 29956-29967.
Kolthur-Seetharam U, Pradeepa MM, Gupta N, Narayanaswamy R and Rao MRS (2009). Spatiotemporal Organization of AT- and GC-rich DNA and Their Association With Transition Proteins TP1 and TP2 in Rat Condensing Spermatids. SAGE Publications Journal of Histochemistry & Cytochemistry vol. 57, (10) 951-962.
2008
Pradeepa MM, Manjunatha S, Sathish V, Agrawal S and Rao MRS (2008). Involvement of Importin-4 in the Transport of Transition Protein 2 into the Spermatid Nucleus. Taylor & Francis Molecular and Cellular Biology vol. 28, (13) 4331-4341.
2007
Pradeepa MM and Rao MRS (2007). Chromatin remodeling during mammalian spermatogenesis: role of testis specific histone variants and transition proteins. Society of Reproduction and Fertility vol. 63, 1-10.
Dhar S, Pradeepa MM and Rao MRS (2007). Emerging concepts of chromatin remodeling in modulating genome functions. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES INDIA SECTION B-BIOLOGICAL SCIENCES vol. 77, 61-71.
Grants
Role of BRD4 mutations in neurodevelopment
Pradeepa Madapura Marulasiddappa
£759,321 MRC Medical Research Council (01-11-2023 - 31-10-2026)
Pradeepa Madapura Marulasiddappa
£759,321 MRC Medical Research Council (01-11-2023 - 31-10-2026)
Role of BRD4 mutations in neurodevelopment
Pradeepa Madapura Marulasiddappa and Nicolae Radu Zabet
£205,822 Barts and the London Charity (01-04-2023 - 31-03-2027)
Pradeepa Madapura Marulasiddappa and Nicolae Radu Zabet
£205,822 Barts and the London Charity (01-04-2023 - 31-03-2027)
Deciphering the role of the MOF complex and H4K16ac in the regulation of neuronal enhancers
Pradeepa Madapura Marulasiddappa
£821,014 MRC Medical Research Council (01-02-2020 - 31-10-2023)
Pradeepa Madapura Marulasiddappa
£821,014 MRC Medical Research Council (01-02-2020 - 31-10-2023)