Dr Ryan O'Shaughnessy

Reader in Cell Biology

FMD/Blizard/Centre for Cell Biology and Cutaneous Research
Queen Mary University of London

r.f.l.oshaughnessy@qmul.ac.uk

www.qmul.ac.uk/blizard/all-staff/profiles/ryan-oshaughnessy.html

Research
Publications
Grants

Research

Epidermal biology, Epidermal barrier function, Ichthyosis, Squamous Cell Carcinoma, Wound healing, Scarring

Cancer Models, Skin Models

Interests

I have a interest in using in vitro models to model disease states and also healthy skin to determine disease mechanisms and also mechanisms important to skin homeostasis. Current relevant expertise is in organotypic, skin equivalent culture, using shRNA knockdown to model human disease.

Publications

2025

Adding arginine to the list of single amino acids important in epidermal barrier function
O’Shaughnessy RFL
British Journal of Dermatology, Oxford University Press (Oup) vol. 193 (1), 7-8.

DOI 10.1093/bjd/ljaf110

25-03-2025

2024

195 Reduced filaggrin expression reduces ERK signalling and disrupts desmosomes in atopic eczema
Hughes A, Coppock L, Tachie-Menson T, Bjorn T, Doykov I, MIlls K, O’Toole EA, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 144 (12)

DOI 10.1016/j.jid.2024.10.202

01-12-2024

P038 Impact of cumulative low-level ozone on keratinocytes and implications for atopic eczema pathogenesis
Tawfik S, Thomas B, Caley M, Jones E, O’Shaughnessy R, O’Toole E
British Journal of Dermatology, Oxford University Press (Oup) vol. 191 (Supplement_1), i31-i32.

DOI 10.1093/bjd/ljae090.065

28-06-2024

P15 Understanding the role of cathepsin H in atopic dermatitis pathogenesis
Tachie-Menson T, Hughes A, O’Toole E, O’Shaughnessy R
British Journal of Dermatology, Oxford University Press (Oup) vol. 190 (6), e85-e86.

DOI 10.1093/bjd/ljae105.037

17-05-2024

Loss-of-function FLG mutations are associated with reduced history of acne vulgaris in a cohort of patients with atopic eczema of Bangladeshi ancestry in East London
Hughes AJ, Barbosa E, Cernova J, Thomas BR, O’Shaughnessy RFL, O’Toole EA
Clinical and Experimental Dermatology, Oxford University Press (Oup) vol. 49 (12), 1547-1553.

DOI 10.1093/ced/llae185

QMRO

15-05-2024

2023

Editorial: Novel aspects of the immunological and structural barrier of the epidermis
O’Shaughnessy R, Common J, Gutowska-Owsiak D
Frontiers in Molecular Biosciences, Frontiers vol. 10

DOI 10.3389/fmolb.2023.1324920

QMRO

01-11-2023

Why are squamous cancers so aggressive in recessive dystrophic epidermolysis bullosa? Can we find new therapeutic approaches?
O’Shaughnessy RFL
British Journal of Dermatology, Oxford University Press (Oup) vol. 190 (1), 7-8.

DOI 10.1093/bjd/ljad392

QMRO

13-10-2023

O14 Junctional epidermolysis bullosa: repairing the epidermal lipid barrier
Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath J, O’Toole E, Caley M
British Journal of Dermatology, Oxford University Press (Oup) vol. 189 (1), e10-e10.

DOI 10.1093/bjd/ljad174.014

07-07-2023

P27 Uncovering the roles of lysosomal protease cathepsin H in atopic dermatitis
Tachie-Menson T, Azizah A, Hughes A, O'Shaughnessy R
British Journal of Dermatology, Oxford University Press (Oup) vol. 189 (1), e24-e25.

DOI 10.1093/bjd/ljad174.048

07-07-2023

P102 Severe atopic eczema is associated with reduced acne vulgaris in Bangladeshi children and young adults: a retrospective analysis from a cross-sectional study
Hughes A, Barbosa E, Cernova J, Thomas B, O’Toole E, O'Shaughnessy R
British Journal of Dermatology, Oxford University Press (Oup) vol. 188 (Supplement_4)

DOI 10.1093/bjd/ljad113.130

26-06-2023

770 AKT1-dependent protease control of skin barrier function and inflammation in atopic dermatitis
Tachie-Menson T, Azizah A, Hughes A, O'Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 143 (5)

DOI 10.1016/j.jid.2023.03.779

01-05-2023

1157 Ozone as an aggravating factor in atopic eczema
Tawfik SS, Thomas B, Jones E, Caley M, O'Shaughnessy R, O'Toole E
Journal of Investigative Dermatology, Elsevier vol. 143 (5)

DOI 10.1016/j.jid.2023.03.1170

01-05-2023

786 IL-4 stimulates IL-1R2 upregulation in atopic dermatitis keratinocytes
Baruah K, Koh L, Tham K, O'Shaughnessy R, O'Toole E, Common JE
Journal of Investigative Dermatology, Elsevier vol. 143 (5)

DOI 10.1016/j.jid.2023.03.795

01-05-2023

ISID1157 - Ozone as an aggravating factor in atopic eczema
Tawfik S, O'Toole E, Thomas B, Caley M, Jones E, O'Shaughnessy R
, Morressier

DOI 10.26226/m.64199a055cbd02001a87dbfc

29-04-2023

Chronic activation of Toll-like receptor 2 induces an ichthyotic skin phenotype
Tagoe H, Hassan S, Bliss E, Youssef G, Heywood W, Mills K, Harper JI, O’Shaughnessy RFL
British Journal of Dermatology, Oxford University Press (Oup) vol. 189 (1), 91-102.

DOI 10.1093/bjd/ljad095

QMRO

27-03-2023

Ozone as an aggravating factor in atopic eczema
Tawfik SS, Thomas B, Jones E, Caley M, O'Shaughnessy R, O'Toole E

01-01-2023

IL-4 stimulates IL-1R2 upregulation in atopic dermatitis keratinocytes
Baruah K, Koh L, Tham K, O'Shaughnessy R, O'Toole E, Common JE

QMRO

01-01-2023

AKT1-dependent protease control of skin barrier function and inflammation in atopic dermatitis
Tachie-Menson T, Azizah A, Hughes A, O'Shaughnessy R

QMRO

01-01-2023

2022

191 Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier
Jones E, Marsh S, O’Shaughnessy R, Aumailley M, McGrath JA, O’Toole EA, Caley MP
Journal of Investigative Dermatology, Elsevier vol. 142 (12)

DOI 10.1016/j.jid.2022.09.202

01-12-2022

183 Reduced FLG expression in atopic eczema reduces expression of key barrier genes and increases BMP signalling
Hughes AJ, Thomas B, O’Toole EA, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 142 (12)

DOI 10.1016/j.jid.2022.09.194

01-12-2022

174 Defining the composition and function of Lamin ribonuclear bodies during keratinocyte terminal differentiation and nuclear degradation
Wotherspoon DJ, Tachie-Menson T, Rogerson C, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 142 (12)

DOI 10.1016/j.jid.2022.09.185

01-12-2022

144 Carbonylated proteins and Ozone in atopic eczema
Tawfik SS, Thomas B, Jones E, Caley MP, O’Shaughnessy R, O’Toole EA
Journal of Investigative Dermatology, Elsevier vol. 142 (12)

DOI 10.1016/j.jid.2022.09.154

01-12-2022

ESDR144 - Carbonylated proteins and Ozone in atopic eczema
Tawfik S, Thomas B, Jones E, Caley M, O'Shaughnessy R, O'Toole E, Thomas B, Prasad Baruah K
, Morressier

DOI 10.26226/m.62fa0093bf6ce2001924f048

26-09-2022

ESDR174 - Defining the composition and function of Lamin ribonuclear bodies during keratinocyte terminal differentiation and nuclear degradation
Wotherspoon D, Tachie-Menson T, Rogerson C, O'Shaughnessy R
, Morressier

DOI 10.26226/m.62fa0094a32203001a2f3e46

26-09-2022

ESDR191 - Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier
Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath J, O'Toole E, Caley M
, Morressier

DOI 10.26226/m.62fa009529d1b0001b31c454

22-09-2022

Reduced filaggrin expression is associated with increased BMP signalling
Hughes A, Thomas B, O'Toole E, O'Shaughnessy R

QMRO

01-01-2022

Quality of life in Bangladeshi children and families with atopic eczema
Tawfik SS, Thomas B, Robinson EJ, Ahmed R, Begum R, Khanam H, Kelsell D, O'Shaughnessy R et al.

01-01-2022

Characterizing novel Akt substrates in squamous cell carcinoma identified N-myc downstream-regulated gene 2 and iron chelation as a novel treatment modality
Button R, Harwood C, O'Shaughnessy R

01-01-2022

Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation
Rogerson C, Wotherspoon D, O'Shaughnessy R

01-01-2022

Carbonylated proteins as markers of oxidative stress and their association with filaggrin genotype in atopic eczema
Tawfik S, Baruah K, Hughes A, Thomas B, O-Shaughnessy R, Toole EO

01-01-2022

Carbonylated proteins and Ozone in atopic eczema
Tawfik SS, Thomas B, Jones E, Caley MP, O'Shaughnessy R, O'Toole EA

01-01-2022

Reduced FLG expression in atopic eczema reduces expression of key barrier genes and increases BMP signalling
Hughes AJ, Thomas B, O'Toole EA, O'Shaughnessy R

01-01-2022

Junctional Epidermolysis Bullosa:Repairing The Epidermal Skin Barrier
Jones E, Marsh S, O'Shaughnessy R, Aumailley M, McGrath JA, O'Toole EA, Caley MP

01-01-2022

Defining the composition and function of Lamin ribonuclear bodies during keratinocyte terminal differentiation and nuclear degradation
Wotherspoon DJ, Tachie-Menson T, Rogerson C, O'Shaughnessy R

01-01-2022

2021

Understanding the paradoxical proinflammatory effects of an immunosuppressant
O’Shaughnessy RFL
British Journal of Dermatology, Oxford University Press (Oup) vol. 185 (6), 1091-1092.

DOI 10.1111/bjd.20778

08-11-2021

282 Phosphoproteomic profiling of AKT-related changes in cutaneous squamous cell carcinoma identified DNA-dependent protein kinase (DNA-PK) and N-Myc downstream regulator 2 (NDRG2) as therapeutic vulnerabilities
Button R, Harwood C, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 141 (10)

DOI 10.1016/j.jid.2021.08.288

01-10-2021

111 Single cell RNA seq revealed altered cytoskeletal dynamics and the formation of cytoplasmic RNA-Lamin A bodies during keratinocyte terminal differentiation
Wotherspoon DJ, Rogerson C, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 141 (10)

DOI 10.1016/j.jid.2021.08.114

01-10-2021

132 Increased BMP Signalling Correlates with Increased Eczema Severity
Hughes AJ, Thomas B, O’Toole EA, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 141 (10)

DOI 10.1016/j.jid.2021.08.135

QMRO

01-10-2021

Ceramide kinase‐like protein, ‘the kinase that isn’t’, finds a role in skin cancer stress relief
O’Shaughnessy RFL
British Journal of Dermatology, Oxford University Press (Oup) vol. 185 (1), 14-15.

DOI 10.1111/bjd.19888

23-03-2021

Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation
Wotherspoon D, Tommasi C, O'Shaughnessy R, Button R, Rogerson C
Cell Death and Differentiation, Springer Nature [Academic Journals On Nature.Com]

DOI 10.1038/s41418-020-00712-9

QMRO

18-01-2021

Increased BMP Signalling Correlates with Increased Eczema Severity
Hughes AJ, Thomas B, O'Toole EA, O'Shaughnessy R

01-01-2021

Phosphoproteomic profiling of AKT-related changes in cutaneous squamous cell carcinoma identified DNA-dependent protein kinase (DNA-PK) and N-Myc downstream regulator 2 (NDRG2) as therapeutic vulnerabilities
Button R, Harwood C, O'Shaughnessy R

QMRO

01-01-2021

Single cell RNA seq revealed altered cytoskeletal dynamics and the formation of cytoplasmic RNA-Lamin A bodies during keratinocyte terminal differentiation
Wotherspoon DJ, Rogerson C, O'Shaughnessy R

01-01-2021

2020

Tape Strips in Dermatology Research.
Hughes AJ, Tawfik SS, Baruah KP, O'Toole EA, O'Shaughnessy RFL
Br J Dermatol

DOI 10.1111/bjd.19760

QMRO

28-12-2020

Perspective: Controlling epidermal terminal differentiation with transcriptional bursting and RNA bodies
O'Shaughnessy R, Rogerson C, Wotherspoon D
Journal of Developmental Biology, Mdpi Ag vol. 8 (4)

DOI 10.3390/jdb8040029

QMRO

04-12-2020

Ubiquitin, a novel therapeutic target in psoriasis?
O'Shaughnessy R
British Journal of Dermatology, Wiley

DOI 10.1111/bjd.19332

QMRO

21-07-2020

2019

Understanding actinic keratosis to reduce squamous cell carcinoma incidence.
O'Shaughnessy RFL
British Journal of Dermatology, Wiley

DOI 10.1111/bjd.18529

QMRO

16-10-2019

PLCD1 and Pilar Cysts
Shimomura Y, O’Shaughnessy R, Rajan N
Journal of Investigative Dermatology, Elsevier vol. 139 (10), 2075-2077.

DOI 10.1016/j.jid.2019.05.027

01-10-2019

Sonic Hedgehog-signalling limits chronic dermatitis via Gli2-driven immune regulatory function
O'Shaughnessy R, Papaioannou E
Journal of Clinical Investigation, American Society For Clinical Investigation

DOI 10.1172/JCI125170

QMRO

02-07-2019

341 Epidermal nuclear degradation during terminal differentiation involves cytoplasmic dispersal of lamina proteins and rapid nuclear shrinkage
Rogerson C, O'Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 139 (5)

DOI 10.1016/j.jid.2019.03.417

QMRO

01-05-2019

Targeting tryptophan transport and breakdown in basal cell carcinoma.
O'Shaughnessy RFL
Br J Dermatol vol. 180 (1), 16-17.

DOI 10.1111/bjd.17316

QMRO

01-01-2019

2018

Differential expression of secreted factors SOSTDC1 and ADAMTS8 cause profibrotic changes in linear morphoea fibroblasts
O'SHAUGHNESSY RFL
British Journal of Dermatology, Wiley

DOI 10.1111/bjd.17352

QMRO

26-10-2018

Protein kinases involved in epidermal barrier formation: The AKT family and other animals.
Rogerson C, O'Shaughnessy RF
Exp Dermatol

DOI 10.1111/exd.13696

QMRO

30-05-2018

909 Kallikrein 6 (KLK6) mediated keratin 10 degradation is required for VZV propagation in skin
Tommasi C, Breuer J, O'Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 138 (5)

DOI 10.1016/j.jid.2018.03.921

QMRO

01-05-2018

2017

Uncovering mechanisms of nuclear degradation in keratinocytes: A paradigm for nuclear degradation in other tissues.
Rogerson C, Bergamaschi D, O'Shaughnessy RFL
Nucleus, 1-9.

DOI 10.1080/19491034.2017.1412027

QMRO

05-12-2017

Phosphoinositide 3-Kinase-Dependent Signalling Pathways in Cutaneous Squamous Cell Carcinomas
Janus JM, O'Shaughnessy RFL, Harwood CA, Maffucci T
Cancers, Mdpi vol. 9 (7)

DOI 10.3390/cancers9070086

QMRO

11-07-2017

A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis
Naeem AS, Tommasi C, Cole C, Brown SJ, Zhu Y, Way B, Willis Owen SAG, Moffatt M et al.
Journal of Allergy and Clinical Immunology vol. 139 (4), 1228-1241.

DOI 10.1016/j.jaci.2016.09.052

QMRO

01-04-2017

CD161⁺ Tconv and CD161⁺ treg share a transcriptional and functional phenotype despite limited overlap in TCRβ repertoire
Duurland CL, Brown CC, O'Shaughnessy RFL, Wedderburn LR
Frontiers in Immunology vol. 8 (MAR)

DOI 10.3389/fimmu.2017.00103

QMRO

06-03-2017

Persistent kallikrein 5 activation induces atopic dermatitis-like skin architecture independent of PAR2 activity
Zhu Y, Underwood J, Macmillan D, Shariff L, O'Shaughnessy R, Harper JI, Pickard C, Friedmann PS et al.
Journal of Allergy and Clinical Immunology, Elsevier vol. 140 (5), 1310-1322.e5.

DOI 10.1016/j.jaci.2017.01.025

QMRO

24-02-2017

2016

Epidermal autophagy and nucleophagy
Akinduro O, Harwood CA, O'Shaughnessy R, Bergamaschi D
European Society of Investigative Dermatology.

DOI 10.1016/j.jid.2016.06.149

QMRO

14-09-2016

393 KLK6-mediated down-regulation of Keratin10 is commonly employed by skin-tropic viruses to propagate in skin and is required for blister formation in VZV infection
Tommasi C, Depledge D, Duddy D, Jones M, Pfister H, Akgul B, Breuer J, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 136 (9)

DOI 10.1016/j.jid.2016.06.413

01-09-2016

429 Defining a common mechanism that leads to scaling in Autosomal Recessive Congenital Ichthyoses
Tagoe H, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 136 (9)

DOI 10.1016/j.jid.2016.06.449

01-09-2016

565 A novel role for the ETS family transcription factor ERG in hypertrophic scarring
Way B, Brown S, Bulstrode N, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 136 (9)

DOI 10.1016/j.jid.2016.06.589

01-09-2016

108 Targeting the toll-like receptor pathway to treat scaling in the autosomal recessive congenital ichthyoses
Hassan S, Tagoe H, Youssef G, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 136 (9)

DOI 10.1016/j.jid.2016.06.126

01-09-2016

Constitutive Autophagy and Nucleophagy during Epidermal Differentiation
Akinduro O, Sully K, Patel A, Robinson DJ, Chikh A, McPhail G, Braun KM, Philpott MP et al.
Journal of Investigative Dermatology vol. 136 (7), 1460-1470.

DOI 10.1016/j.jid.2016.03.016

QMRO

01-07-2016

483 MDM2 binds and ubiquitinates keratin 10 in VZV infection and is required for viral propagation and blister formation
Tomassi C, Depledge D, Otto R, Tan W, Duddy D, Breuer J, O’Shaughnessy R
Journal of Investigative Dermatology, Elsevier vol. 136 (5)

DOI 10.1016/j.jid.2016.02.520

01-05-2016

Mosaic Activating Mutations in GNA11 and GNAQ Are Associated with Phakomatosis Pigmentovascularis and Extensive Dermal Melanocytosis
Thomas AC, Zeng Z, Rivière J-B, O’Shaughnessy R, Al-Olabi L, St.-Onge J, Atherton DJ, Aubert H et al.
Journal of Investigative Dermatology, Elsevier vol. 136 (4), 770-778.

DOI 10.1016/j.jid.2015.11.027

QMRO

14-01-2016

2015

AKT1-mediated Lamin A/C degradation is required for nuclear degradation and normal epidermal terminal differentiation
Naeem AS, Zhu Y, Di WL, Marmiroli S, O'Shaughnessy RFL
Cell Death & Differentiation, Springer Nature vol. 22 (12), 2123-2132.

DOI 10.1038/cdd.2015.62

QMRO

05-06-2015

Insight from the Air–Skin Interface
O'Shaughnessy RFL, Brown SJ
Journal of Investigative Dermatology, Elsevier vol. 135 (2), 331-333.

DOI 10.1038/jid.2014.457

01-02-2015

2014

A novel microdeletion in LOR causing autosomal dominant loricrin keratoderma
Kinsler VA, Drury S, Khan A, Waelchli R, Rukaite G, Barnicoat A, Lench N, Harper JI et al.
British Journal of Dermatology, Oxford University Press (Oup) vol. 172 (1), 262-264.

DOI 10.1111/bjd.13361

QMRO

09-12-2014

CD161+ regulatory t cells in immune regulation
Duurland C, Brooks T, Hubank M, O'Shaughnessy R, Wedderburn L
Pediatric Rheumatology, Springer Nature vol. 12 (Suppl 1)

DOI 10.1186/1546-0096-12-s1-p39

01-09-2014

2013

PReS-FINAL-1021: Further characterization of CD161+ regulatory t cells in health and disease
Duurland C, Chain G, O'Shaughnessy R, Pesenacker A, Wedderburn L
Pediatric Rheumatology, Springer Nature vol. 11 (Suppl 2)

DOI 10.1186/1546-0096-11-s2-p17

01-12-2013

The protein phosphatase 2A regulatory subunit Ppp2r2a is required for Connexin‐43 dephosphorlyation during epidermal barrier acquisition
Gerner L, Youssef G, O'Shaughnessy RFL
Experimental Dermatology, Wiley vol. 22 (11), 754-756.

DOI 10.1111/exd.12234

29-10-2013

Identifying a Hyperkeratosis Signature in Autosomal Recessive Congenital Ichthyosis: Mdm2 Inhibition Prevents Hyperkeratosis in a Rat ARCI Model
Youssef G, Ono M, Brown SJ, Kinsler VA, Sebire NJ, Harper JI, O'Shaughnessy RFL
Journal of Investigative Dermatology, Elsevier vol. 134 (3), 858-861.

DOI 10.1038/jid.2013.374

QMRO

04-09-2013

Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition
Youssef G, Gerner L, Naeem AS, Ralph O, Ono M, O’Neill CA, O’Shaughnessy RFL
Developmental Biology, Elsevier vol. 380 (2), 274-285.

DOI 10.1016/j.ydbio.2013.04.034

16-05-2013

T-Cell Reconstitution after Thymus Xenotransplantation Induces Hair Depigmentation and Loss
Furmanski AL, O'Shaughnessy RFL, Saldana JI, Blundell MP, Thrasher AJ, Sebire NJ, Davies EG, Crompton T
Journal of Investigative Dermatology, Elsevier vol. 133 (5), 1221-1230.

DOI 10.1038/jid.2012.492

QMRO

10-01-2013

Constitutive autophagy as a terminal differentiation mechanism
Akinduro O, Sully K, Chikh A, O'shaughnessy RF, Bergamaschi D, Harwood CA, Philpott MP, Byrne C

01-01-2013

2012

Interleukin-1 alpha blockade prevents hyperkeratosis in an in vitro model of lamellar ichthyosis
O'Shaughnessy RFL, Chaudhury I, Harper JI
Human Molecular Genetics, Oxford University Press (Oup) vol. 22 (5), 1059-1059.

DOI 10.1093/hmg/dds511

18-12-2012

The mTOR inhibitor rapamycin opposes carcinogenic changes to epidermal Akt1/PKBα isoform signaling
Sully K, Akinduro O, Philpott MP, Naeem AS, Harwood CA, Reeve VE, O'Shaughnessy RF, Byrne C
Oncogene, Springer Nature vol. 32 (27), 3254-3262.

DOI 10.1038/onc.2012.338

13-08-2012

Autophagy in epidermis
Akinduro O, Sully K, O'Shaughnessy RFL, Harwood CA, Bergamaschi D, Byrne C

01-01-2012

AKT1 Loss Correlates with Episomal HPV16 in Vulval Intraepithelial Neoplasia
Ekeowa-Anderson AL, Purdie KJ, Gibbon K, Byrne CR, Arbeit JM, Harwood CA, O'Shaughnessy RFL
Plos One, Public Library of Science (Plos) vol. 7 (6)

DOI 10.1371/journal.pone.0038608

QMRO

01-01-2012

A Novel Mechanism of Keratinocyte Terminal Differentiation - Autophagy?
Akinduro O, Sully K, O'Shaughnessy R, Harwood CA, Bergamaschi D, Byrne C

01-01-2012

2011

TALE homeodomain proteins regulate site-specific terminal differentiation, LCE genes and epidermal barrier
Jackson B, Brown SJ, Avilion AA, O'Shaughnessy RFL, Sully K, Akinduro O, Murphy M, Cleary ML et al.
Development vol. 138 (11), 1681-1690.
01-06-2011

TALE homeodomain proteins regulate site-specific terminal differentiation, LCE genes and epidermal barrier.
Jackson B, Brown SJ, Avilion AA, O'Shaughnessy RFL, Sully K, Akinduro O, Murphy M, Cleary ML et al.
J Cell Sci vol. 124 (Pt 10), 1681-1690.

DOI 10.1242/jcs.077552

15-05-2011

Understanding the tumour-suppressing role of rapamycin in epidermis
Sully K, Akinduro O, O'Shaughnessy RF, Harwood CA, Byrne C

11-04-2011

Rapamycin treatment increases mTORC2 activity and selectively activates the AKT1 isoform in epidermis by inhibition of the mTORC1-dependent feedback loop to IRS-1
Sully K, Akinduro OA, Philpott MP, Harwood CA, O'Shaughnessy RF, Byrne C

01-01-2011

2010

Interleukin-1 alpha blockade prevents hyperkeratosis in an in vitro model of lamellar ichthyosis
O'Shaughnessy RFL, Choudhary I, Harper JI
Human Molecular Genetics, Oxford University Press (Oup) vol. 19 (13), 2594-2605.

DOI 10.1093/hmg/ddq145

12-04-2010

Whole-mount assays for gene induction and barrier formation in the developing epidermis.
Byrne C, Avilion AA, O'Shaughnessy RF, Welti JC, Hardman MJ
Methods Mol Biol vol. 585, 271-286.

DOI 10.1007/978-1-60761-380-0_19

QMRO

01-01-2010

2009

Akt-dependent Pp2a activity is required for epidermal barrier formation during late embryonic development.
O'Shaughnessy RFL, Welti JC, Sully K, Byrne C
Development vol. 136 (20), 3423-3431.

DOI 10.1242/dev.037010

QMRO

01-10-2009

AKT-dependent protein phosphatase 2A activity is necessary for epidermal barrier function and the correct formation of the cornified layer
O'Shaughnessy RFL, Welti JC, Byrne CR

01-04-2009

2008

Akt-dependent, protein phosphatase 2A-mediated formation of tight junctions is an initial component of the epidermal permeability barrier
O'Shaughnessy RFL, Welti JC, Byrne CR

01-04-2008

2007

Cutaneous human papillomaviruses down-regulate AKT1, whereas AKT2 up-regulation and activation associates with tumors.
O'Shaughnessy RFL, Akgũl B, Storey A, Pfister H, Harwood CA, Byrne C
Cancer Res vol. 67 (17), 8207-8215.

DOI 10.1158/0008-5472.CAN-07-0755

01-09-2007

AKT-dependent HspB1 (Hsp27) activity in epidermal differentiation.
O'Shaughnessy RFL, Welti JC, Cooke JC, Avilion AA, Monks B, Birnbaum MJ, Byrne C
J Biol Chem vol. 282 (23), 17297-17305.

DOI 10.1074/jbc.M610386200

08-06-2007

Cutaneous human papillomavirus early genes downregulate serine phosphorylated Akt1 while upregulating Akt2 expression
O'Shaughnessy RFL, Akgul B, Storey A, Pfister H, Harwood CA, Byrne C

01-05-2007

Epidermal AKT signalling regulates SREBP transcription, epidermal lipogenesis and skin barrier activity
Welti JC, O'Shaughnessy RFL, Byrne C

01-05-2007

2006

Cutaneous HPV early genes target epidermal AKT activity, altering heat-shock protein regulation of cornification
O'Shaughnessy R, Welti J, Pfister H, Harwood C, Byrne C

01-08-2006

2004

The WNT signalling modulator, Wise, is expressed in an interaction-dependent manner during hair-follicle cycling.
O'Shaughnessy RFL, Yeo W, Gautier J, Jahoda CAB, Christiano AM
J Invest Dermatol vol. 123 (4), 613-621.

DOI 10.1111/j.0022-202X.2004.23410.x

01-10-2004

The role of BMP signalling in the control of ID3 expression in the hair follicle
O'Shaughnessy RFL, Christiano AM, Jahoda CAB
Experimental Dermatology, Wiley vol. 13 (10), 621-629.

DOI 10.1111/j.0906-6705.2004.00206.x

24-09-2004

The lanceolate hair rat phenotype results from a missense mutation in a calcium coordinating site of the desmoglein 4 gene
Jahoda CAB, Kljuic A, O'Shaughnessy R, Crossley N, Whitehouse CJ, Robinson M, Reynolds AJ, Demarchez M et al.
Genomics, Elsevier vol. 83 (5), 747-756.

DOI 10.1016/j.ygeno.2003.11.015

01-05-2004

Inherited disorders of the skin in human and mouse: from development to differentiation.
O'Shaughnessy RFL, Christiano AM
The International Journal of Developmental Biology, Upv/Ehu Press vol. 48 (2-3), 171-179.

DOI 10.1387/ijdb.15272382

01-01-2004

2003

Desmoglein 4 in Hair Follicle Differentiation and Epidermal Adhesion Evidence from Inherited Hypotrichosis and Acquired Pemphigus Vulgaris
Kljuic A, Bazzi H, Sundberg JP, Martinez-Mir A, O'Shaughnessy R, Mahoney MG, Levy M, Montagutelli X et al.
Cell, Elsevier vol. 113 (2), 249-260.

DOI 10.1016/s0092-8674(03)00273-3

01-04-2003

2001

Analysis of Gene Expression Using High-Density and IFN-γ-Specific Low-Density cDNA Arrays
Aberger F, Costa-Pereira AP, Schlaak JF, Williams TM, O'Shaughnessy RFL, Hollaus G, Kerr IM, Frischauf A-MM
Genomics, Elsevier vol. 77 (1-2), 50-57.

DOI 10.1006/geno.2001.6623

01-09-2001

Stem Cells in the Epidermis
O’Shaughnessy RFL, Christiano AM
Skin Pharmacology and Physiology, Karger Publishers vol. 14 (6), 350-357.

DOI 10.1159/000056368

01-01-2001

2000

PA‐FABP, a novel marker of human epidermal transit amplifying cells revealed by 2D protein gel electrophoresis and cDNA array hybridisation
O'Shaughnessy RFL, Seery JP, Celis JE, Frischauf A-M, Watt FM
Febs Letters, Wiley vol. 486 (2), 149-154.

DOI 10.1016/s0014-5793(00)02252-3

07-12-2000

Orchestrated control of filaggrin-actin scaffolds underpins cornification
Danuta G-O, de la Serna J, Fritzsche M, Naeem A, Podobas E, Leeming M, Colin-York H, O'SHAUGHNESSY R et al.
Cell Death and Disease, Nature Publishing Group

DOI 10.1038/s41419-018-0407-2

QMRO

Kallikrein-mediated cytokeratin 10 degradation is required from VZV propagation in skin
O'Shaughnessy R
Journal of Investigative Dermatology, Elsevier

DOI 10.1016/j.jid.2019.08.448

QMRO

Akt1-associated actomyosin remodelling is required for nuclear lamina dispersal and nuclear shrinkage in epidermal terminal differentiation
Rogerson C, Wotherspoon D, O’Shaughnessy RFL
In Biorxiv

DOI 10.1101/868034

Chronic activation of Toll-like receptor 2 induces an ichthyotic skin phenotype
Tagoe H, Hassan S, Youssef G, Heywood W, Mills K, Harper JI, O’Shaughnessy RFL
In Biorxiv

DOI 10.1101/2022.06.06.494922

Phosphoproteomic analysis of the AKT signalling axis in cutaneous squamous carcinoma progression reveals novel therapeutic targets
Button R, Harwood C, O’Shaughnessy R
In Biorxiv

DOI 10.1101/2022.10.03.510591

Loss of cholesterol in Junctional Epidermolysis Bullosa skin identifies a key role for Laminin-332 in actomyosin mediated cholesterol transport
Jones EM, Camera E, Parzymies P, Marsh ST, O’Shaughnessy RF, Aumailley M, McGrath JA, O’Toole EA et al.
In Biorxiv

DOI 10.1101/2023.09.10.557030

Grants

Investigating mTOR inhibition by Lactobacillus Rhamnosus lysates to treat atopic dermatitis
Ryan O'Shaughnessy
£9,778 British Skin Foundation (BSF)
01-08-2025 - 31-07-2026

Understanding the epidermal functions of JAK/STAT signalling in atopic dermatitis
Ryan O'Shaughnessy and Edel O'Toole
£80,839 British Skin Foundation (BSF)
01-12-2023 - 30-11-2025

BSID Undergraduate Research Experience in Summer 2024
Ryan O'Shaughnessy
£2,000 British Skin Foundation (BSF)
08-07-2024 - 07-09-2024

Phosphoproteomic identification of key AKT kinases and AKT targets for prevention and treatment of Squamous Cell Carcinoma - consumables only
Ryan O'Shaughnessy and Catherine Harwood
£9,962 British Skin Foundation (BSF)
04-02-2021 - 03-02-2022

AKT1-dependent control of barrier function and inflammation in eczema
Ryan O'Shaughnessy and Edel O'Toole
£236,971 Barts and the London Charity
01-07-2020 - 31-12-2024

Understanding the Regulation of Epidermal Differentiation Genes by Filaggrin in Eczema
Ryan O'Shaughnessy and Edel O'Toole
£273,043 Medical Research Council
17-02-2020 - 30-04-2023

Understanding mechanisms of AKT expression and activity during skin cancer progression
Ryan O'Shaughnessy
£211,574 Barts and the London Charity
01-02-2018 - 20-01-2025