Dr Tom Nightingale
Senior Lecturer
The William Harvey Research Institute
Queen Mary University of London
Queen Mary University of London
Research
Intracellular Trafficking, Endothelial cells, Haemostasis, Inflammation, Weibel Palade Bodies, Cell Junctions
Interests
My interests are in vitro models of the vasculature. We hope to use these to closer investigate the intracellular trafficking events associated with inflammation and blood clotting. Upon endothelial stimulation clotting factors and inflammatory receptors are mobilised from intracellular stores. These are essential for recruiting platelets and leukocytes to sites of injury. We are also interested in understanding how junctions remodel to allow increased permeability and leukocyte transmigration. The vasculature is composed of a number of different cell types and is constantly exposed to flow. This is difficult to model using standard culture models.Publications
2025
Endothelial β-PIX (ARHGEF7) drives exocytosis through enabling the dynamic reorganisation of the cytoskeletonEl-Mansi S, Deegan KM, Donker L, Godinho S, Nightingale TD
In Cold Spring Harbor Laboratory
07-05-2025
Probing rare von Willebrand disease–causing mutations in the D4 and C-domains of von Willebrand factorMobayen G, El-Mansi S, Chion A, Nightingale TD, McKinnon TAJ
Research and Practice in Thrombosis and Haemostasis, Elsevier Bv vol. 9 (4), 102922-102922.
01-05-2025
2024
Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membraneEl-Mansi S, Mitchell TP, Mobayen G, McKinnon TAJ, Miklavc P, Frick M, Nightingale TD
Blood Advances, American Society of Hematology vol. 8 (17), 4714-4726.
10-09-2024
2023
The unconventional Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membraneEl-Mansi S, Mitchell TP, Miklavc P, Frick M, Nightingale TD
In Cold Spring Harbor Laboratory
12-08-2023
Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flowBosseboeuf E, Chikh A, Chaker AB, Mitchell TP, Vignaraja D, Rajendrakumar R, Khambata RS, Nightingale TD et al.
Science Signaling, American Association For The Advancement of Science (Aaas) vol. 16 (786)
23-05-2023
Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factorEl-Mansi S, Robinson CL, Kostelnik KB, McCormack JJ, Mitchell TP, Lobato-Márquez D, Rajeeve V, Cutillas P et al.
Blood, American Society of Hematology vol. 141 (8), 930-944.
23-02-2023
2022
Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescenceCarpintero-Fernández P, Borghesan M, Eleftheriadou O, Pan-Castillo B, Fafián-Labora JA, Mitchell TP, Yuste A, Ogrunc M et al.
Cell Death &Amp; Disease, Springer Science and Business Media Llc vol. 13 (2)
19-02-2022
2021
The coagulation factor IX (F9) loss of function prevents the cell cycle arrest induced by CDK4/6 inhibitors treatmentCarpintero-Fernández P, Borghesan M, Eleftheriadou O, Fafián-Labora JA, Mitchell TP, Nightingale TD, Mayán MD, O’Loghlen A
In Cold Spring Harbor Laboratory
30-11-2021
Proximity interactome of LC3B in normal growth conditionsNollet M, Agrotis A, Michailidis F, Dokal AD, Rajeeve V, Burden J, Nightingale TD, Cutillas P et al.
In Cold Spring Harbor Laboratory
09-10-2021
Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammationReglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS et al.
Immunity, Elsevier Bv vol. 54 (9), 1989-2004.e9.
01-09-2021
Emerging mechanisms to modulate VWF release from endothelial cellsEl-Mansi S, Nightingale TD
The International Journal of Biochemistry &Amp; Cell Biology, Elsevier Bv vol. 131, 105900-105900.
01-02-2021
2020
Trafficking of JAM-C is regulated by cytoplasmic tail motifs and is essential to endothelial cell migrationMitchell TP, Kostelnik KB, Barker A, Schultz C, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S et al.
01-01-2020
2019
Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migrationKostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S et al.
Plos Biology, Public Library of Science (Plos) vol. 17 (12)
02-12-2019
NMDA receptor modulation of glutamate release in activated neutrophilsdel Arroyo AG, Hadjihambi A, Sanchez J, Turovsky E, Kasymov V, Cain D, Nightingale TD, Lambden S et al.
Ebiomedicine, Elsevier Bv vol. 47, 457-469.
01-09-2019
2018
Tuning the endothelial response: differential release of exocytic cargos from Weibel‐Palade bodiesNightingale TD, McCormack JJ, Grimes W, Robinson C, Lopes da Silva M, White IJ, Vaughan A, Cramer LP et al.
Journal of Thrombosis and Haemostasis, Elsevier Bv vol. 16 (9), 1873-1886.
01-09-2018
Decrease in Von-Willebrand factor protein in the endothelium of human coronary atherosclerotic plaques: Possible mechanisms and role in thrombosisTarvala U, El Matary R, Nightingale T, Poston R
01-08-2018
F-actin and septin coordinate to drive the exocytosis of a pro-haemostatic molecule from endothelial cellsRobinson CL, McCormack JJ, White IJ, Cutler DF, Nightingale TD
01-01-2018
2017
Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodiesStevenson NL, White IJ, McCormack JJ, Robinson C, Cutler DF, Nightingale TD
Journal of Cell Science, The Company of Biologists vol. 130 (15), 2591-2605.
01-08-2017
2016
JAM‐C deficiency primes endothelial cells for a pro‐inflammatory stateReglero N, Beal R, Cabrera C, Pickworth C, Golding M, Whiteford J, Nightingale T, Imhof B et al.
01-04-2016
2013
The secretion of von Willebrand factor from endothelial cells; an increasingly complicated storyNightingale T, Cutler D
Journal of Thrombosis and Haemostasis, Elsevier Bv vol. 11, 192-201.
01-06-2013
2012
Actin coats and rings promote regulated exocytosisNightingale TD, Cutler DF, Cramer LP
Trends in Cell Biology, Elsevier Bv vol. 22 (6), 329-337.
01-06-2012
2011
Actomyosin II contractility expels von Willebrand factor from Weibel–Palade bodies during exocytosisNightingale TD, White IJ, Doyle EL, Turmaine M, Harrison-Lavoie KJ, Webb KF, Cramer LP, Cutler DF
Journal of Cell Biology, Rockefeller University Press vol. 194 (4), 613-629.
15-08-2011
Myosin Va Acts in Concert with Rab27a and MyRIP to Regulate Acute Von‐Willebrand Factor Release from Endothelial CellsRojo Pulido I, Nightingale TD, Darchen F, Seabra MC, Cutler DF, Gerke V
Traffic, Wiley vol. 12 (10), 1371-1382.
05-08-2011
Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood. 2009;113(20):5010–5018.Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF
Blood, American Society of Hematology vol. 117 (12), 3476-3476.
24-03-2011
A role for Rab10 in von Willebrand factor release discovered by an AP‐1 interactor screen in C. elegansMICHAUX G, DYER CEF, NIGHTINGALE TD, GALLAUD E, NURRISH S, CUTLER DF
Journal of Thrombosis and Haemostasis, Elsevier Bv vol. 9 (2), 392-401.
01-02-2011
2009
Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cellsNightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF
Blood, American Society of Hematology vol. 113 (20), 5010-5018.
14-05-2009
A Mechanism of Sialylation Functionally Silences the Hyaluronan Receptor LYVE-1 in Lymphatic EndotheliumNightingale TD, Frayne MEF, Clasper S, Banerji S, Jackson DG
J Biol Chem vol. 284 (6), 3935-3945.
06-02-2009
2008
Aftiphilin and γ-Synergin Are Required for Secretagogue Sensitivity of Weibel-Palade Bodies in Endothelial CellsLui-Roberts WWY, Ferraro F, Nightingale TD, Cutler DF
Molecular Biology of The Cell, American Society For Cell Biology (Ascb) vol. 19 (12), 5072-5081.
01-12-2008
Formation and function of Weibel-Palade bodiesMetcalf DJ, Nightingale TD, Zenner HL, Lui-Roberts WW, Cutler DF
Journal of Cell Science, The Company of Biologists vol. 121 (1), 19-27.
01-01-2008
2005
Functional regulation of the lymphatic hyaluronan receptor (LYVE-1) by terminal sialic acid.Nightingale TD, Banerji S, Jackson DG
01-01-2005
Dynamic trafficking and turnover of Jam-C is essential for endothelial cell migrationKostelnik KB, Barker A, Schultz C, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S, Cutillas P et al.
In Biorxiv
Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissuesRolas L, Stein M, Barkaway A, Reglero N, Sciacca E, Yaseen M, Wang H, Vazquez-Martinez L et al.
Embo Reports, Springer
Grants
The role of endothelial storage granule formation and release in senescenceTom Nightingale and Loic Rolas
£549,924 Medical Research Council
01-10-2025 - 30-09-2028
Novel Regulatory Mechanisms for VWF secretion: Towards Therapeutic ControlTom Nightingale and Mathieu-Benoit Voisin
£286,032 BHF British Heart Foundation
01-02-2023 - 31-01-2026
Understanding the role of Syndecan-4 in neovascular eye diseaseJames Whiteford and Tom Nightingale
£323,174 Barts and the London Charity
01-12-2022 - 30-09-2027
Advancing Mesenchymal Stromal Cell-Dressing Therapy for Myocardial RepairKen Suzuki, Tom Nightingale, Mauro Perretti, Federica Marelli-Berg, Michael Barnes, Anthony Mathur and Fiona Lewis
£1,263,590 BHF British Heart Foundation
01-09-2020 - 31-05-2026
New approaches to limit angiogenic responses by controlling endothelial junctional remodellingTom Nightingale and James Whiteford
£233,773 Barts and the London Charity
01-09-2020 - 31-01-2024
Controlling the haemostatic response: Novel regulatory mechanisms for Von Willebrand factor secretion from endothelial cellsTom Nightingale
£232,656 BHF British Heart Foundation
01-02-2020 - 02-11-2023
Using a pathway dynamics approach to identify novel EP4 receptor agonists for the treatment of cardiac inflammation and fibrosis (iCASE matching)Peter McCormick, Lesley Howell and Tom Nightingale
£107,727 Barts and the London Charity
01-10-2019 - 30-09-2023
Investigation into novelregulatory mechanism for Von Willebrand factor secretion from endothelial cellsTom Nightingale
£200,752 BHF British Heart Foundation
18-04-2016 - 17-04-2019
The role of Jam-king in endothelial vascular functionTom Nightingale
£389,244 MRC Medical Research Council
14-09-2015 - 13-10-2018