Dr Tom Nightingale
Senior Lecturer
The William Harvey Research Institute
Queen Mary University of London
Queen Mary University of London
Research
Intracellular Trafficking, Endothelial cells, Haemostasis, Inflammation, Weibel Palade Bodies, Cell Junctions
Interests
My interests are in vitro models of the vasculature. We hope to use these to closer investigate the intracellular trafficking events associated with inflammation and blood clotting. Upon endothelial stimulation clotting factors and inflammatory receptors are mobilised from intracellular stores. These are essential for recruiting platelets and leukocytes to sites of injury. We are also interested in understanding how junctions remodel to allow increased permeability and leukocyte transmigration. The vasculature is composed of a number of different cell types and is constantly exposed to flow. This is difficult to model using standard culture models.Publications
2024
Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membraneEl-Mansi S, Mitchell TP, Mobayen G, McKinnon TAJ, Miklavc P, Frick M and Nightingale TD
Blood Advances, American Society of Hematology vol. 8 (17), 4714-4726.
10-09-2024
Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissuesRolas L, Stein M, Barkaway A, Reglero-Real N, Sciacca E, Yaseen M, Wang H, Vazquez-Martinez L, Golding M, Blacksell IA, Giblin MJ, Jaworska E, Bishop CL, Voisin M-B, Gaston-Massuet C, Fossati-Jimack L, Pitzalis C, Cooper D, Nightingale TD, Lopez-Otin C, Lewis MJ and Nourshargh S
Embo Reports, Springer Nature vol. 25 (9), 3842-3869.
25-06-2024
2023
Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flowBosseboeuf E, Chikh A, Chaker AB, Mitchell TP, Vignaraja D, Rajendrakumar R, Khambata RS, Nightingale TD, Mason JC, Randi AM, Ahluwalia A and Raimondi C
Science Signaling, American Association For The Advancement of Science (Aaas) vol. 16 (786)
23-05-2023
2022
Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factorEl-Mansi S, Robinson CL, Kostelnik KB, McCormack JJ, Mitchell TP, Lobato-Márquez D, Rajeeve V, Cutillas P, Cutler DF, Mostowy S and Nightingale TD
Blood, American Society of Hematology vol. 141 (8), 930-944.
26-12-2022
Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescenceCarpintero-Fernández P, Borghesan M, Eleftheriadou O, Pan-Castillo B, Fafián-Labora JA, Mitchell TP, Yuste A, Ogrunc M, Nightingale TD, Mayan M and O’Loghlen A
Cell Death & Disease, Springer Nature vol. 13 (2)
19-02-2022
2021
Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammationReglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS, Gonzalez-Nuñez M, Austin-Williams SN, Cooper D, Vázquez-Martínez L, Fu T, De Rossi G, Golding M, Voisin M-B, Boulanger CM, Kubota Y, Muller WA, Tooze SA, Nightingale TD, Collinson L, Perretti M, Aksoy E and Nourshargh S
Immunity, Elsevier vol. 54 (9), 1989-2004.e9.
06-08-2021
2020
Emerging mechanisms to modulate VWF release from endothelial cellsEl-Mansi S and Nightingale TD
The International Journal of Biochemistry & Cell Biology, Elsevier vol. 131
07-12-2020
2019
Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migrationKostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S, Cutillas P and Nightingale TD
Plos Biology, Public Library of Science (Plos) vol. 17 (12)
02-12-2019
NMDA receptor modulation of glutamate release in activated neutrophilsdel Arroyo AG, Hadjihambi A, Sanchez J, Turovsky E, Kasymov V, Cain D, Nightingale TD, Lambden S, Grant SGN, Gourine AV and Ackland GL
Ebiomedicine, Elsevier Bv
01-08-2019
2018
Tuning the endothelial response: differential release of exocytic cargos from Weibel-Palade Bodies.NIGHTINGALE TD, McCormack JJ, Grimes W, ROBINSON C, Lopes da Silva M, White IJ, Vaughan A, Cramer LP and Cutler DF
Journal of Thrombosis and Haemostasis, Wiley
28-06-2018
2017
Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies.NIGHTINGALE TD, CUTLER DF, Robinson C, McCormack JJ, White IJ and Stevenson NL
, Editors: Schiavo G. Journal of Cell Science, Company of Biologists
05-07-2017
2013
The secretion of von Willebrand factor from endothelial cells; an increasingly complicated storyNightingale T and Cutler D
Journal of Thrombosis and Haemostasis, Elsevier vol. 11 (Suppl 1), 192-201.
01-06-2013
2012
Actin coats and rings promote regulated exocytosisNightingale TD, Cutler DF and Cramer LP
Trends in Cell Biology, Elsevier vol. 22 (6), 329-337.
28-04-2012
2011
Myosin Va Acts in Concert with Rab27a and MyRIP to Regulate Acute Von‐Willebrand Factor Release from Endothelial CellsPulido IR, Nightingale TD, Darchen F, Seabra MC, Cutler DF and Gerke V
Traffic, Wiley vol. 12 (10), 1371-1382.
05-08-2011
Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood. 2009;113(20):5010–5018.Nightingale TD, Pattni K, Hume AN, Seabra MC and Cutler DF
Blood, American Society of Hematology vol. 117 (12)
24-03-2011
A role for Rab10 in von Willebrand factor release discovered by an AP‐1 interactor screen in C. elegansMICHAUX G, DYER CEF, NIGHTINGALE TD, GALLAUD E, NURRISH S and CUTLER DF
Journal of Thrombosis and Haemostasis, Elsevier vol. 9 (2), 392-401.
27-01-2011
Actomyosin II contractility expels von Willebrand
factor from Weibel–Palade bodies during exocytosis.NIGHTINGALE TD, White IJ, Doyle EL, Turmaine M, Harrison-Lavoie KJ, Webb KF, Cramer LP and Cutler DF
J Cell Biol. vol. 194 (4), 613-629.
01-01-2011
2009
Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells.NIGHTINGALE TD, Pattni K, Hume AN, Seabra MC and Cutler DF
Blood vol. 113 (20), 5010-5018.
01-01-2009
2008
A Mechanism of Sialylation Functionally Silences the Hyaluronan Receptor LYVE-1 in Lymphatic Endothelium*Nightingale TD, Frayne MEF, Clasper S, Banerji S and Jackson DG
Journal of Biological Chemistry, Elsevier vol. 284 (6), 3935-3945.
25-11-2008
Aftiphilin and γ-Synergin Are Required for Secretagogue Sensitivity of Weibel-Palade Bodies in Endothelial CellsLui-Roberts WWY, Ferraro F, Nightingale TD and Cutler DF
Molecular Biology of The Cell, American Society For Cell Biology (Ascb) vol. 19 (12), 5072-5081.
24-09-2008
Formation and function of Weibel-Palade bodiesMetcalf DJ, Nightingale TD, Zenner HL, Lui-Roberts WW and Cutler DF
Journal of Cell Science, The Company of Biologists vol. 121 (1), 19-27.
01-01-2008
Grants
Novel Regulatory Mechanisms for VWF secretion: Towards Therapeutic ControlTom Nightingale and Mathieu-Benoit Voisin
£286,032 BHF British Heart Foundation
01-02-2023 - 31-01-2026
Understanding the role of Syndecan-4 in neovascular eye diseaseJames Whiteford and Tom Nightingale
£323,174 Barts and the London Charity
01-12-2022 - 30-09-2026
Advancing Mesenchymal Stromal Cell-Dressing Therapy for Myocardial RepairKen Suzuki, Tom Nightingale, Mauro Perretti, Federica Marelli-Berg, Michael Barnes, Anthony Mathur and Fiona Lewis
£1,263,590 BHF British Heart Foundation
01-09-2020 - 31-08-2025
New approaches to limit angiogenic responses by controlling endothelial junctional remodellingTom Nightingale and James Whiteford
£233,773 Barts and the London Charity
01-09-2020 - 31-01-2024
Controlling the haemostatic response: Novel regulatory mechanisms for Von Willebrand factor secretion from endothelial cellsTom Nightingale
£232,656 BHF British Heart Foundation
01-02-2020 - 02-11-2023
Using a pathway dynamics approach to identify novel EP4 receptor agonists for the treatment of cardiac inflammation and fibrosis (iCASE matching)Peter McCormick, Lesley Howell and Tom Nightingale
£107,727 Barts and the London Charity
01-10-2019 - 30-09-2023
Investigation into novelregulatory mechanism for Von Willebrand factor secretion from endothelial cellsTom Nightingale
£200,752 BHF British Heart Foundation
18-04-2016 - 17-04-2019
The role of Jam-king in endothelial vascular functionTom Nightingale
£389,244 MRC Medical Research Council
14-09-2015 - 13-10-2018