Dr Tom Nightingale
Senior Lecturer
The William Harvey Research Institute
Queen Mary University of London
Queen Mary University of London
Research
Intracellular Trafficking, Endothelial cells, Haemostasis, Inflammation, Weibel Palade Bodies, Cell Junctions
Interests
My interests are in vitro models of the vasculature. We hope to use these to closer investigate the intracellular trafficking events associated with inflammation and blood clotting. Upon endothelial stimulation clotting factors and inflammatory receptors are mobilised from intracellular stores. These are essential for recruiting platelets and leukocytes to sites of injury. We are also interested in understanding how junctions remodel to allow increased permeability and leukocyte transmigration. The vasculature is composed of a number of different cell types and is constantly exposed to flow. This is difficult to model using standard culture models.Publications
2024
El-Mansi S, Mitchell TP, Mobayen G, McKinnon TAJ, Miklavc P, Frick M and Nightingale TD (2024). Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane. American Society of Hematology Blood Advances vol. 8, (17) 4714-4726. Rolas L, Stein M, Barkaway A, Reglero-Real N, Sciacca E, Yaseen M, Wang H, Vazquez-Martinez L, Golding M, Blacksell IA, Giblin MJ, Jaworska E, Bishop CL, Voisin M-B, Gaston-Massuet C, Fossati-Jimack L, Pitzalis C, Cooper D, Nightingale TD, Lopez-Otin C, Lewis MJ and Nourshargh S (2024). Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissues. Springer Nature EMBO Reports vol. 25, (9) 3842-3869. 2023
Bosseboeuf E, Chikh A, Chaker AB, Mitchell TP, Vignaraja D, Rajendrakumar R, Khambata RS, Nightingale TD, Mason JC, Randi AM, Ahluwalia A and Raimondi C (2023). Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flow. American Association for the Advancement of Science (AAAS) Science Signaling vol. 16, (786) 2022
El-Mansi S, Robinson CL, Kostelnik KB, McCormack JJ, Mitchell TP, Lobato-Márquez D, Rajeeve V, Cutillas P, Cutler DF, Mostowy S and Nightingale TD (2022). Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factor. American Society of Hematology Blood vol. 141, (8) 930-944. Carpintero-Fernández P, Borghesan M, Eleftheriadou O, Pan-Castillo B, Fafián-Labora JA, Mitchell TP, Yuste A, Ogrunc M, Nightingale TD, Mayan M and O’Loghlen A (2022). Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescence. Springer Nature Cell Death & Disease vol. 13, (2) 2021
Reglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS, Gonzalez-Nuñez M, Austin-Williams SN, Cooper D, Vázquez-Martínez L, Fu T, De Rossi G, Golding M, Voisin M-B, Boulanger CM, Kubota Y, Muller WA, Tooze SA, Nightingale TD, Collinson L, Perretti M, Aksoy E and Nourshargh S (2021). Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation. Elsevier Immunity vol. 54, (9) 1989-2004.e9. 2020
El-Mansi S and Nightingale TD (2020). Emerging mechanisms to modulate VWF release from endothelial cells. Elsevier The International Journal of Biochemistry & Cell Biology vol. 131, 2019
Kostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S, Cutillas P and Nightingale TD (2019). Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration. Public Library of Science (PLoS) PLOS Biology vol. 17, (12) del Arroyo AG, Hadjihambi A, Sanchez J, Turovsky E, Kasymov V, Cain D, Nightingale TD, Lambden S, Grant SGN, Gourine AV and Ackland GL (2019). NMDA receptor modulation of glutamate release in activated neutrophils. Elsevier BV EBioMedicine 2018
NIGHTINGALE TD, McCormack JJ, Grimes W, ROBINSON C, Lopes da Silva M, White IJ, Vaughan A, Cramer LP and Cutler DF (2018). Tuning the endothelial response: differential release of exocytic cargos from Weibel-Palade Bodies. Wiley Journal of Thrombosis and Haemostasis 2017
NIGHTINGALE TD, CUTLER DF, Robinson C, McCormack JJ, White IJ and Stevenson NL (2017). Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies., Editors: Schiavo G. Company of Biologists Journal of Cell Science 2013
Nightingale T and Cutler D (2013). The secretion of von Willebrand factor from endothelial cells; an increasingly complicated story. Elsevier Journal of Thrombosis and Haemostasis vol. 11, (Suppl 1) 192-201. 2012
Nightingale TD, Cutler DF and Cramer LP (2012). Actin coats and rings promote regulated exocytosis. Elsevier Trends in Cell Biology vol. 22, (6) 329-337. 2011
Pulido IR, Nightingale TD, Darchen F, Seabra MC, Cutler DF and Gerke V (2011). Myosin Va Acts in Concert with Rab27a and MyRIP to Regulate Acute Von‐Willebrand Factor Release from Endothelial Cells. Wiley Traffic vol. 12, (10) 1371-1382. Nightingale TD, Pattni K, Hume AN, Seabra MC and Cutler DF (2011). Nightingale TD, Pattni K, Hume AN, Seabra MC, Cutler DF. Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood. 2009;113(20):5010–5018. American Society of Hematology Blood vol. 117, (12) MICHAUX G, DYER CEF, NIGHTINGALE TD, GALLAUD E, NURRISH S and CUTLER DF (2011). A role for Rab10 in von Willebrand factor release discovered by an AP‐1 interactor screen in C. elegans. Elsevier Journal of Thrombosis and Haemostasis vol. 9, (2) 392-401. NIGHTINGALE TD, White IJ, Doyle EL, Turmaine M, Harrison-Lavoie KJ, Webb KF, Cramer LP and Cutler DF (2011). Actomyosin II contractility expels von Willebrand
factor from Weibel–Palade bodies during exocytosis. J Cell Biol. vol. 194, (4) 613-629. 2009
NIGHTINGALE TD, Pattni K, Hume AN, Seabra MC and Cutler DF (2009). Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. Blood vol. 113, (20) 5010-5018. 2008
Nightingale TD, Frayne MEF, Clasper S, Banerji S and Jackson DG (2008). A Mechanism of Sialylation Functionally Silences the Hyaluronan Receptor LYVE-1 in Lymphatic Endothelium*. Elsevier Journal of Biological Chemistry vol. 284, (6) 3935-3945. Lui-Roberts WWY, Ferraro F, Nightingale TD and Cutler DF (2008). Aftiphilin and γ-Synergin Are Required for Secretagogue Sensitivity of Weibel-Palade Bodies in Endothelial Cells. American Society for Cell Biology (ASCB) Molecular Biology of the Cell vol. 19, (12) 5072-5081. Metcalf DJ, Nightingale TD, Zenner HL, Lui-Roberts WW and Cutler DF (2008). Formation and function of Weibel-Palade bodies. The Company of Biologists Journal of Cell Science vol. 121, (1) 19-27. Grants
Novel Regulatory Mechanisms for VWF secretion: Towards Therapeutic ControlThomas Nightingale and Mathieu-Benoit Voisin
£286,032 BHF British Heart Foundation (01-02-2023 - 31-01-2026)
New approaches to limit angiogenic responses by controlling endothelial junctional remodellingThomas Nightingale and James Whiteford
£233,773 Barts and the London Charity (01-09-2020 - 31-01-2024)
Controlling the haemostatic response: Novel regulatory mechanisms for Von Willebrand factor secretion from endothelial cellsThomas Nightingale
£232,656 BHF British Heart Foundation (01-02-2020 - 02-11-2023)